Edinburgh Research Explorer

H3K9me maintenance on a Human Artificial Chromosome is required for 3 segregation but not centromere epigenetic memory

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Open Access permissions

Open

Documents

  • Download as Adobe PDF

    Final published version, 19.9 MB, PDF document

    Licence: Creative Commons: Attribution (CC-BY)

Original languageEnglish
Article numberjcs242610
JournalJournal of Cell Science
Volume133
DOIs
Publication statusPublished - 24 Jul 2020

Abstract

Most eukaryotic centromeres are located within heterochromatic regions.Paradoxically, heterochromatin can also antagonize de novo centromere formation and some centromeres lack it altogether. In order to investigate the importance of heterochromatin at centromeres, we used epigenetic engineering of a syntheticalphoidtetO Human Artificial Chromosome (HAC), to which chimeric proteins can betargeted. By tethering the JMJD2D demethylase, we removed heterochromatin markH3K9me3 specifically from the HAC centromere. This caused no short-term defects,but long-term tethering reduced HAC centromere protein levels and triggered HACmis-segregation. Yet, centromeric CENP-A was maintained at a reduced level.Furthermore, HAC centromere function was compatible with an alternative low-H3K9me3, high-H3K27me3 chromatin signature, as long as residual levels of H3K9me3 remained. When JMJD2D was released from the HAC, H3K9me3 levels recovered over several days back to initial levels along with CENP-A/-C and mitotic segregation fidelity. Our results suggest that a minimal level of heterochromatin is required to stabilize mitotic centromere function but not for maintaining centromere epigenetic memory, and that a homeostatic pathway maintains heterochromatin at centromeres.

    Research areas

  • centromere, heterochromatin, polycomb, mitosis, kinetochore, CENP-A, human artificial chromosome, epigenetic engineering

Download statistics

No data available

ID: 152057245