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Licence: Creative Commons: Attribution (CC-BY)
Original language | English |
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Pages (from-to) | 226-238 |
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Journal | Genes & Development |
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Volume | 34 |
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Issue number | 3-4 |
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Early online date | 9 Jan 2020 |
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DOIs | |
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Publication status | E-pub ahead of print - 9 Jan 2020 |
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Centromeres are maintained epigenetically by the presence of CENP-A, an evolutionarily conserved histone H3 variant, which directs kinetochore assembly and hence centromere function. To identify factors that promote assembly of CENP-A chromatin, we affinity-selected solubilized fission yeast CENP-ACnp1 chromatin. All subunits of the Ino80 complex were enriched, including the auxiliary subunit Hap2. Chromatin association of Hap2 is Ies4-dependent. In addition to a role in maintenance of CENP-ACnp1 chromatin integrity at endogenous centromeres, Hap2 is required for de novo assembly of CENP-ACnp1 chromatin on naïve centromere DNA and promotes H3 turnover on centromere regions and other loci prone to CENP-ACnp1 deposition. Prior to CENP-ACnp1 chromatin assembly, Hap2 facilitates transcription from centromere DNA. These analyses suggest that Hap2–Ino80 destabilizes H3 nucleosomes on centromere DNA through transcription-coupled histone H3 turnover, driving the replacement of resident H3 nucleosomes with CENP-ACnp1 nucleosomes. These inherent properties define centromere DNA by directing a program that mediates CENP-ACnp1 assembly on appropriate sequences.
- CENP-A, centromere, chromatin, chromosome segregation, epigenetic, fission yeast, histone, kintectochore, remodeling, transcription
ID: 129972664