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Hepatic Progenitor Specification from Pluripotent Stem Cells Using a Defined Differentiation System

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  • Jose Meseguer-Ripolles, Yu Wang, Agnes Sorteberg, Aishwariya Sharma, Nan-Linda Ding, Baltasar Lucendo-Villarin, Philipp Kramer, Charis-Patricia Segeritz, David C. Hay

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  • Meseguer-Ripolles et al 2020 JoVE

    Accepted author manuscript, 68.4 KB, Word document

Original languageEnglish
JournalJournal of Visualized Experiments (JoVE)
Publication statusAccepted/In press - 3 Mar 2020


Liver disease is an escalating global health issue. While liver transplantation is an effective mode of therapy, patient mortality has increased due to shortages in donor organ availability. Organ scarcity also affects the routine supply of human hepatocytes for basic research and the clinic. Therefore, the development of renewable sources of human liver progenitor cells is desirable and is the goal of this study. To be able to effectively generate and deploy human liver progenitors on a large scale, a reproducible hepatic progenitor differentiation system was developed that can be used with both human embryonic and induced pluripotent stem cell lines. This protocol aids experimental reproducibility between users in a range of cell cultureware formats. These are important advantages over current differentiation systems that will enhance the basic research and may pave the way towards clinical product development.

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