Edinburgh Research Explorer

Highly selective and rapidly activatable fluorogenic Thrombin sensors and application in human lung tissue

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Open Access permissions

Open

Documents

  • Download as Adobe PDF

    Final published version, 2.1 MB, PDF document

    Licence: Creative Commons: Attribution (CC-BY)

http://xlink.rsc.org/?DOI=C7OB00663B
Original languageEnglish
JournalOrganic & Biomolecular chemistry
Early online date28 Apr 2017
DOIs
Publication statusE-pub ahead of print - 28 Apr 2017

Abstract

A library of FRET-based peptides were prepared and studied as Thrombin substrates. This identified probes that showed selective activation by Thrombin, low fluorescent background signals, stability to Factor Xa, matrix metalloproteases, and primary human inflammatory cell lysates and supernatant. These were selected for further optimization, creating a second generation of fluorogenic probes with improved solubility and Plasmin resistance. The optimised probe allowed the detection of Thrombin activity in ex vivo fibrotic human tissue.

Download statistics

No data available

ID: 35863656