Research output: Contribution to journal › Article › peer-review
Rights statement: Open Access funded by Biotechnology and Biological Sciences Research Council Under a Creative Commons license
Final published version, 470 KB, PDF document
Original language | English |
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Pages (from-to) | 106-113 |
Number of pages | 8 |
Journal | Current Opinion in Microbiology |
Volume | 19 |
Issue number | 1 |
DOIs | |
Publication status | Published - 14 Jul 2014 |
A revolution in sequencing technologies in recent years has led to dramatically increased throughput and reduced cost of bacterial genome sequencing. An increasing number of applications of the new technologies are providing broad insights into bacterial evolution, epidemiology, and pathogenesis. For example, the capacity to sequence large numbers of bacterial isolates is enabling high resolution phylogenetic analyses of bacterial populations leading to greatly enhanced understanding of the emergence, adaptation, and transmission of pathogenic clones. In addition, RNA-seq offers improved quantification and resolution for transcriptomic analysis, and the combination of high-throughput sequencing with transposon mutagenesis is a powerful approach for the identification of bacterial determinants required for survival in vivo. In this concise review we provide selected examples of how high throughput sequencing is being applied to understand the biology of bacterial pathogens, and discuss future technological advances likely to have a profound impact on the field.
ID: 16507222