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Human axial progenitors generate trunk neural crest cells in vitro

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    Rights statement: Copyright Frith et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Original languageEnglish
Number of pages27
JournaleLIFE
Volume7
DOIs
Publication statusPublished - 10 Aug 2018

Abstract

The neural crest (NC) is a multipotent embryonic cell population that generates distinct cell types in an axial position-dependent manner. The production of NC cells from human pluripotent stem cells (hPSCs) is a valuable approach to study human NC biology. However, the origin of human trunk NC remains undefined and current in vitro differentiation strategies induce only a modest yield of trunk NC cells. Here we show that hPSC-derived axial progenitors, the posteriorly-located drivers of embryonic axis elongation, give rise to trunk NC cells and their derivatives. Moreover, we define the molecular signatures associated with the emergence of human NC cells of distinct axial identities in vitro. Collectively, our findings indicate that there are two routes toward a human post-cranial NC state: the birth of cardiac and vagal NC is facilitated by retinoic acid-induced posteriorisation of an anterior precursor whereas trunk NC arises within a pool of posterior axial progenitors.

    Research areas

  • In vitro differentiation, axial progenitors, developmental biology, embryonic development, human, neural crest, neuromesodermal progenitors, pluripotent stem cells, regenerative medicine, stem cells

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