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Identification of Novel Avian Influenza Virus Derived CD8+T-Cell Epitopes

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  • S.S.N. Reemers
  • D.A. van Haarlem
  • A.J.A.M. Sijts
  • L. Vervelde
  • C.A. Jansen

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http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0031953
Original languageEnglish
Article numbere31953
Number of pages10
JournalPLoS ONE
Volume7
Issue number2
DOIs
Publication statusPublished - 23 Feb 2012

Abstract

Avian influenza virus (AIV) infection is a continuing threat to both humans and poultry. Influenza virus specific CD8+ T cells are associated with protection against homologous and heterologous influenza strains. In contrast to what has been described for humans and mice, knowledge on epitope-specific CD8+ T cells in chickens is limited. Therefore, we set out to identify AIV-specific CD8+ T-cell epitopes. Epitope predictions based on anchor residues resulted in 33 candidate epitopes. MHC I inbred chickens were infected with a low pathogenic AIV strain and sacrificed at 5, 7, 10 and 14 days post infection (dpi). Lymphocytes isolated from lung, spleen and blood were stimulated ex vivo with AIV-specific pooled or individual peptides and the production of IFN gamma was determined by ELIspot. This resulted in the identification of 12 MHC B12-restricted, 3 B4-restricted and 1 B19-restricted AIV-specific CD8+ T-cell epitopes. In conclusion, we have identified novel AIV-derived CD8+ T-cell epitopes for several inbred chicken strains. This knowledge can be used to study the role of CD8+ T cells against AIV infection in a natural host for influenza, and may be important for vaccine development.

    Research areas

  • IMMUNITY, MAJOR HISTOCOMPATIBILITY COMPLEX, T-CELL RESPONSES, MEMORY, CLASS-I MOLECULE, CHICKEN, INFECTION, HEMAGGLUTININ, MHC, A VIRUS

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