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Improving TCSPC data acquisition from CMOS SPAD arrays

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Original languageEnglish
Publication statusPublished - 17 Jun 2013
EventSPIE European Conference on Biomedical Optics - Munich, Germany
Duration: 17 Jun 201321 Feb 2014


ConferenceSPIE European Conference on Biomedical Optics


We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from
32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of
TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over
a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We
characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function,
time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen
(Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds
(with average timestamp acquisition rate of 4.7 million counts per second).


SPIE European Conference on Biomedical Optics


Munich, Germany

Event: Conference

ID: 13303397