Edinburgh Research Explorer

In Vitro Assays to Measure Histone Methyltransferase Activity Using Different Chromatin Substrates

Research output: Chapter in Book/Report/Conference proceedingChapter

Related Edinburgh Organisations

Open Access permissions

Open

Documents

Original languageEnglish
Title of host publicationPlant Chromatin Dynamics
EditorsMarian Bemer, Célia Baroux
PublisherHUMANA PRESS INC
Pages345-360
Number of pages16
Volume1675
Edition1st ed. 2018 edition
ISBN (Print)1493973177, 9781493973170
DOIs
Publication statusPublished - 20 Oct 2017

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1675
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Abstract

In vitro histone modification (HM) assays are used to characterize the activity of chromatin-modifying enzymes. These assays provide information regarding the modification sites on histones, the product specificity, and the impact of other histone or nucleotide modifications on enzyme activity. In particular, histone methyltransferase (HMT) assays have been instrumental in elucidating the activity and site specificity of many plant HMT enzymes. In this chapter, we describe a general protocol that can be used to perform HMT assays using different chromatin substrates, detection methods, and enzymes directly purified from plant material or heterologous sources.

    Research areas

  • Histone modifications, histone variants, histone lysine methylation, histone peptides, histone octamers, nucleosomes

Download statistics

No data available

ID: 57525192