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Inhibition of infectious bursal disease virus infection by artificial microRNAs targeting chicken heat-shock protein 90

Research output: Contribution to journalArticle

  • Weifeng Yuan
  • Xinyu Zhang
  • Xiaoli Xia
  • Huaichang Sun

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Original languageEnglish
Pages (from-to)876-9
Number of pages4
JournalJournal of General Virology
Volume93
Issue numberPt 4
DOIs
Publication statusPublished - Apr 2012

Abstract

Infectious bursal disease virus (IBDV) causes an important disease in young chickens. Chicken heat-shock protein 90 (cHsp90) has been shown to be a functional component of the cellular receptor complex for IBDV infection. This study demonstrates the inhibitory effect of vector-expressed anti-cHsp90α microRNA (miRNA) on IBDV infection. The reporter vectors pcHsp90α-EGFP and pcHsp90β-EGFP were constructed to facilitate effective miRNA selection. Two anti-cHsp90α and one anti-cHsp90β miRNA-expression vectors were constructed for a stable transfection study. Poly(A)-tailed RT-PCR detected sequence-specific miRNA transcription in transfected cells. Semiquantitative RT-PCR showed inhibition of cHsp90 transcription in transfected cells. A virus-titration assay showed that the anti-cHsp90α miRNA, but not the anti-cHsp90β miRNA, had inhibitory effects on IBDV infection. These results suggest that cHsp90α is a functional component of the cellular receptor complex for IBDV infection, and that anti-cHsp90α miRNA could be used as an anti-IBDV reagent.

    Research areas

  • Animals, Birnaviridae Infections, Cell Line, Chickens, HSP90 Heat-Shock Proteins, Infectious bursal disease virus, MicroRNAs, Polymerase Chain Reaction, Poultry Diseases, Receptors, Virus, Transcription, Genetic, Transfection

ID: 13077565