Edinburgh Research Explorer

Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Open Access permissions

Open

Documents

  • Download as Adobe PDF

    Rights statement: Copyright Booth et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

    Final published version, 4 MB, PDF-document

Original languageEnglish
Article numbere01641
JournaleLIFE
Volume2014
Issue number3
DOIs
Publication statusPublished - 27 May 2014

Abstract

When the nucleolus disassembles during open mitosis, many nucleolar proteins and RNAs associate with chromosomes, establishing a perichromosomal compartment coating the chromosome periphery. At present nothing is known about the function of this poorly characterised compartment. In this study, we report that the nucleolar protein Ki-67 is required for the assembly of the perichromosomal compartment in human cells. Ki-67 is a cell-cycle regulated protein phosphatase 1-binding protein that is involved in phospho-regulation of the nucleolar protein B23/nucleophosmin. Following siRNA depletion of Ki-67, NIFK, B23, nucleolin, and four novel chromosome periphery proteins all fail to associate with the periphery of human chromosomes. Correlative light and electron microscopy (CLEM) images suggest a near-complete loss of the entire perichromosomal compartment. Mitotic chromosome condensation and intrinsic structure appear normal in the absence of the perichromosomal compartment but significant differences in nucleolar reassembly and nuclear organisation are observed in post-mitotic cells.

    Research areas

  • Cell Nucleolus, Chromosomes, Human, HeLa Cells, Humans, Intracellular Signaling Peptides and Proteins, Ki-67 Antigen, Microscopy, Electron, Mitosis, Molecular Sequence Data, Nuclear Proteins, Phosphoproteins, Phosphorylation, Protein Phosphatase 1, RNA Interference, RNA-Binding Proteins, Sequence Analysis, Protein, Signal Transduction, Transfection

Download statistics

No data available

ID: 16662352