Edinburgh Research Explorer

Loss of functional Dicer in mouse radial glia cell-autonomously prolongs cortical neurogenesis

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Open Access permissions

Open

Documents

  • Download as Adobe PDF

    Rights statement: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

    Final published version, 5 MB, PDF document

    Licence: Creative Commons: Attribution (CC-BY)

http://www.sciencedirect.com/science/article/pii/S001216061300448X
Original languageEnglish
Pages (from-to)530-537
Number of pages8
JournalDevelopmental Biology
Volume382
Issue number2
DOIs
Publication statusPublished - 15 Oct 2013

Abstract

Radial glia of the mouse cerebral cortex emerge from neuroepithelial stem cells around embryonic day 11 and produce excitatory cortical neurons until a few days before birth. The molecular mechanisms that regulate the end of cortical neurogenesis remain largely unknown. Here we investigated if the Dicer-dependent microRNA (miRNA) pathway is involved. By electroporating a cre-recombinase expression vector into the cortex of E13.5 embryos carrying a conditional allele of Dicer1, we induced mosaic recombination causing Dicer1 deletion and reporter activation in a subset of radial glia. We analysed the long-term fates of their progeny. We found that mutant radial glia produced abnormally large numbers of Cux1-positive neurons, many of which populated the superficial cortical layers. Injections of the S-phase marker bromodeoxyuridine between postnatal days 3 and 14 showed that much of this population was generated postnatally. Our findings suggest a role for Dicer-dependent processes in limiting the timespan of cortical neurogenesis.

    Research areas

  • Dicer, microRNA, Neurogenesis, Telencephalon, Mouse

Download statistics

No data available

ID: 10459910