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Low-Latency Time-Resolved Fluorescence Lifetime Flow Cytometry and Cell Sorting using a CMOS Silicon Photomultiplier

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Original languageEnglish
Number of pages1
Publication statusPublished - 26 Jun 2015
EventCongress of International Society for the Advancement of Cytometry (CYTO) - Glasgow, United Kingdom
Duration: 26 Jun 201530 Sep 2015

Conference

ConferenceCongress of International Society for the Advancement of Cytometry (CYTO)
CountryUnited Kingdom
CityGlasgow
Period26/06/1530/09/15

Abstract

Accurate and fast measurement of fluorescence lifetime in flow cytometry would prompt
application to studies of protein-protein interactions by Forster resonance energy transfer (FRET), which
are otherwise difficult or impossible to analyse [1]. Time-correlated single photon counting (TCSPC) has
the best signal to noise ratio of any fluorescence lifetime technique [2] but cannot readily be implemented
within a cytometer due to the requirement of complex software algorithms, severely restricting the
throughput and preventing real-time sorting of cells [3].
The SiPM is a single-chip silicon photomultiplier detector employing a CMOS single-photon avalanche
diode (SPAD) array light detector and time to digital converters (TDCs) [4]. The sensor's pile-up resistant
embedded centre of mass (CMM) processor accomplishes low-latency measurement and thresholding of
fluorescence lifetime. A digital control signal is generated with a maximum 16.6μs latency for cell sorter
actuation.

Event

Congress of International Society for the Advancement of Cytometry (CYTO)

26/06/1530/09/15

Glasgow, United Kingdom

Event: Conference

ID: 21595855