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Low-Latency Time-Resolved Fluorescence Lifetime Flow Cytometry and Cell Sorting using a CMOS Silicon Photomultiplier

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Original languageEnglish
Number of pages1
Publication statusPublished - 26 Jun 2015
EventCongress of International Society for the Advancement of Cytometry (CYTO) - Glasgow, United Kingdom
Duration: 26 Jun 201530 Sep 2015


ConferenceCongress of International Society for the Advancement of Cytometry (CYTO)
CountryUnited Kingdom


Accurate and fast measurement of fluorescence lifetime in flow cytometry would prompt
application to studies of protein-protein interactions by Forster resonance energy transfer (FRET), which
are otherwise difficult or impossible to analyse [1]. Time-correlated single photon counting (TCSPC) has
the best signal to noise ratio of any fluorescence lifetime technique [2] but cannot readily be implemented
within a cytometer due to the requirement of complex software algorithms, severely restricting the
throughput and preventing real-time sorting of cells [3].
The SiPM is a single-chip silicon photomultiplier detector employing a CMOS single-photon avalanche
diode (SPAD) array light detector and time to digital converters (TDCs) [4]. The sensor's pile-up resistant
embedded centre of mass (CMM) processor accomplishes low-latency measurement and thresholding of
fluorescence lifetime. A digital control signal is generated with a maximum 16.6μs latency for cell sorter


Congress of International Society for the Advancement of Cytometry (CYTO)


Glasgow, United Kingdom

Event: Conference

ID: 21595855