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Making immortalized cell lines from embryonic mouse kidney

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    Accepted author manuscript, 2.06 MB, PDF document

Original languageEnglish
Pages (from-to)165-71
Number of pages7
JournalMethods in Molecular Biology
Publication statusPublished - 2012


Immortalized cell lines derived from embryonic mouse kidneys are useful tools for exploring signaling pathways, morphogenetic mechanisms, and gene function in renal development: they also provide a means for efficient first-round screening of panels of small molecules intended to combat renal pathologies such as the development of cysts, and such cell line-based screening can allow a valuable reduction in the numbers of animals needed for a given line of research. This chapter presents a simple method for generating cell lines from the "Immortomouse," which carries a temperature-sensitive SV40 antigen, under the control of an interferon-regulated promoter.

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