Edinburgh Research Explorer

Making immortalized cell lines from embryonic mouse kidney

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Open Access permissions

Open

Documents

  • Download as Adobe PDF

    Rights statement: "Authors may self-archive the author’s accepted manuscript of their articles on their own websites. Authors may also deposit this version of the article in any repository, provided it is only made publicly available 12 months after official publication or later. He/ she may not use the publisher's version (the final article), which is posted on SpringerLink and other Springer websites, for the purpose of self-archiving or deposit. Furthermore, the author may only post his/her version provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: “The final publication is available at link.springer.com”." http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0

    Accepted author manuscript, 2 MB, PDF document

http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
Original languageEnglish
Pages (from-to)165-71
Number of pages7
JournalMethods in Molecular Biology
Volume886
DOIs
Publication statusPublished - 2012

Abstract

Immortalized cell lines derived from embryonic mouse kidneys are useful tools for exploring signaling pathways, morphogenetic mechanisms, and gene function in renal development: they also provide a means for efficient first-round screening of panels of small molecules intended to combat renal pathologies such as the development of cysts, and such cell line-based screening can allow a valuable reduction in the numbers of animals needed for a given line of research. This chapter presents a simple method for generating cell lines from the "Immortomouse," which carries a temperature-sensitive SV40 antigen, under the control of an interferon-regulated promoter.

Download statistics

No data available

ID: 4094284