Edinburgh Research Explorer

Methods for Precisely Localised Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Open Access permissions

Open

Documents

  • Download as Adobe PDF

    Rights statement: Copyright © 2015 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License

    Final published version, 851 KB, PDF document

    Licence: Creative Commons: Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

Original languageEnglish
Article numbere53295
JournalJournal of Visualized Experiments (JoVE)
Issue number106
DOIs
Publication statusPublished - 25 Dec 2015

Abstract

We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.

Download statistics

No data available

ID: 23325500