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Migration of Chemotactic Bacteria in Soft Agar: Role of Gel Concentration

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)525-534
Number of pages10
JournalBiophysical Journal
Volume101
Issue number3
DOIs
StatePublished - 3 Aug 2011

Abstract

We study the migration of chemotactic wild-type Escherichia colt populations in semisolid (soft) agar in the concentration range C = 0.15-0.5% (w/v). For C less than or similar to 0.35%, expanding bacterial colonies display characteristic chemotactic rings. At C = 0.35%, however, bacteria migrate as broad circular bands rather than sharp rings. These are growth/diffusion waves arising because of suppression of chemotaxis by the agar and have not been previously reported experimentally to our knowledge. For C = 0.4-0.5%, expanding colonies do not span the depth of the agar and develop pronounced front instabilities. The migration front speed is weakly dependent on agar concentration at C < 0.25%, but decreases sharply above this value. We discuss these observations in terms of an extended Keller-Segel model for which we derived novel transport parameter expressions accounting for perturbations of the chemotactic response by collisions with the agar. The model makes it possible to fit the observed front speed decay in the range C= 0.15-0.35%, and its solutions qualitatively reproduce the observed transition from chemotactic to growth/diffusion bands. We discuss the implications of our results for the study of bacteria in porous media and for the design of improved bacteriological chemotaxis assays.

    Research areas

  • ESCHERICHIA-COLI, SALMONELLA-TYPHIMURIUM, TRAVELING BANDS, GROWTH, MODEL, TRANSPORT, POPULATIONS, COLONIES, MOTILITY

ID: 1487091