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Milk Lacking α-Casein Leads to Permanent Reduction in Body Size in Mice

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    Rights statement: Copyright: © 2011 Kolb et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021775
Original languageEnglish
Article numbere21775
JournalPLoS ONE
Volume6
Issue number7
DOIs
StatePublished - 2011

Abstract

The major physiological function of milk is the transport of amino acids, carbohydrates, lipids and minerals to mammalian offspring. Caseins, the major milk proteins, are secreted in the form of a micelle consisting of protein and calcium-phosphate.We have analysed the role of the milk protein α-casein by inactivating the corresponding gene in mice. Absence of α-casein protein significantly curtails secretion of other milk proteins and calcium-phosphate, suggesting a role for α-casein in the establishment of casein micelles. In contrast, secretion of albumin, which is not synthesized in the mammary epithelium, into milk is not reduced. The absence of α-casein also significantly inhibits transcription of the other casein genes. α-Casein deficiency severely delays pup growth during lactation and results in a life-long body size reduction compared to control animals, but has only transient effects on physical and behavioural development of the pups. The data support a critical role for α-casein in casein micelle assembly. The results also confirm lactation as a critical window of metabolic programming and suggest milk protein concentration as a decisive factor in determining adult body weight.

    Research areas

  • Animals, Apoptosis, Behavior, Animal, Biological Markers, Body Size, Body Weight, Calcium, Caseins, Cell Line, Female, Gene Expression Regulation, Gene Targeting, Health, Immunohistochemistry, Mammary Glands, Animal, Mass Spectrometry, Mice, Milk, Models, Biological, Phosphates

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