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Multiobjective optimization of gene circuits for metabolic engineering

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Original languageEnglish
Pages (from-to)13-16
Number of pages4
JournalIFAC-PapersOnLine
Volume52
Issue number26
DOIs
Publication statusPublished - 26 Dec 2019
Event8th IFAC Conference on Foundations of Systems Biology in Engineering: 8th FOSBE 2019 - Universitat Politècnica de València, Valencia, Spain
Duration: 15 Aug 201918 Aug 2019
Conference number: 8
http://fosbe2019.ai2.upv.es/

Abstract

Metabolic engineering has enabled the production of a wealth of chemicals with microorganisms. Classic strategies for pathway engineering rely on the expression of heterologous enzymes in a host that convert native intermediates into target products. Although traditional implementations are based on open-loop control, recent advances in gene circuit engineering offer opportunities for building feedback systems that dynamically control pathway activity. Here we present a framework for the design of metabolic control circuits based on multiobjective optimization. We show that positive and negative feedback loops produce a range of optimal dynamics along a Pareto front. Such regulatory loops define connectivities between pathway intermediates and enzymatic genes that trade-off metabolic production against the burden to the host. Our results lay the groundwork for the automated design of gene circuitry in applications at the interface of synthetic biology and metabolic engineering.

    Research areas

  • Metabolic engineering, Synthetic Biology, multiobjective optimization

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