Edinburgh Research Explorer

Neutron Reflectivity as a Tool for Physics-Based Studies of Model Bacterial Membranes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Related Edinburgh Organisations

Open Access permissions

Open

Documents

http://link.springer.com/chapter/10.1007%2F978-3-319-32189-9_16
Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
Pages261-282
Number of pages22
Volume915
ISBN (Electronic)978-3-319-32189-9
DOIs
StatePublished - 19 May 2016

Abstract

The principles of neutron reflectivity and its application as a tool to provide structural information at the (sub-) molecular unit length scale from models for bacterial membranes are described. The model membranes can take the form of a monolayer for a single leaflet spread at the air/water interface, or bilayers of increasing complexity at the solid/liquid interface. Solid-supported bilayers constrain the bilayer to 2D but can be used to characterize interactions with antimicrobial peptides and benchmark high throughput lab-based techniques. Floating bilayers allow for membrane fluctuations, making the phase behaviour more representative of native membranes. Bilayers of varying levels of compositional accuracy can now be constructed, facilitating studies with aims that range from characterizing the fundamental physical interactions, through to the characterization of accurate mimetics for the inner and outer membranes of Gram-negative bacteria. Studies of the interactions of antimicrobial peptides with monolayer and bilayer models for the inner and outer membranes have revealed information about the molecular control of the outer membrane permeability, and the mode of interaction of antimicrobials with both inner and outer membranes.

Download statistics

No data available

ID: 27398620