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Novel bead-based platform for direct detection of unlabelled nucleic acids through Single Nucleobase Labelling

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http://www.sciencedirect.com/science/article/pii/S0039914016306579
Original languageEnglish
Pages (from-to)489-496
Number of pages8
JournalTalanta
Volume161
Early online date31 Aug 2016
DOIs
Publication statusE-pub ahead of print - 31 Aug 2016

Abstract

Over the last decade, circulating microRNAs have received attention as diagnostic and prognostic biomarkers. In particular, microRNA122 has been demonstrated to be an early and more sensitive indicator of drug-induced liver injury than the widely used biomarkers such as alanine aminotransferase and aspartate aminotransferase. Recently, microRNA122 has been used in vitro to assess the cellular toxicity of new drugs and as a biomarker for the development of a rapid test for drug overdose/liver damage. In this proof-of-concept study, we report a PCR-free and label-free detection method that has a limit of detection (3 standard deviations) of 15 fmoles of microRNA122, by integrating a dynamic chemical approach for "Single Nucleobase Labelling" with a bead-based platform (Luminex(®)) thereby, in principle, demonstrating the exciting prospect of rapid and accurate profiling of any microRNAs related to diseases and toxicology.

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