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Novel Genetic Variants are Associated With Increased Vertebral Volumetric BMD, Reduced Vertebral Fracture Risk, and Increased Expression of SCL1A3 and EPHB2

Research output: Contribution to journalArticle

  • Carrie M Nielson
  • Ching-Ti Liu
  • Albert V Smith
  • Cheryl L Ackert-Bicknell
  • Sjur Reppe
  • Jakobsdottir Johanna
  • Christina Wassel
  • Thomas C Register
  • Ling Oei
  • Edwin H Oei
  • Neeta Parimi
  • Elizabeth J Samelson
  • Mike A Nalls
  • Joseph Zmuda
  • Thomas Lang
  • Mary Bouxsein
  • Jeanne Latourelle
  • Melina Claussnitzer
  • Kristin Siggeirsdottir
  • Priya Srikanth
  • Erik Lorentzen
  • Liesbeth Vandenput
  • Carl Langefeld
  • Laura Raffield
  • Greg Terry
  • Amanda J Cox
  • Matthew A Allison
  • Michael H Criqui
  • Don Bowden
  • M Arfan Ikram
  • Dan Mellström
  • Magnus K Karlsson
  • John Carr
  • Matthew Budoff
  • Caroline Phillips
  • L Adrienne Cupples
  • Wen-Chi Chou
  • Richard H Myers
  • Kaare M Gautvik
  • Peggy M Cawthon
  • Steven Cummings
  • David Karasik
  • Fernando Rivadeneira
  • Vilmundur Gudnason
  • Eric S Orwoll
  • Tamara B Harris
  • Claes Ohlsson
  • Douglas P Kiel
  • Yi-Hsiang Hsu

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Original languageEnglish
JournalJournal of Bone and Mineral Research
Volume31
Issue number12
Early online date1 Aug 2016
DOIs
StatePublished - Dec 2016

Abstract

Genome-wide association studies (GWAS) have revealed numerous loci for areal bone mineral density (aBMD). We completed the first GWAS meta-analysis (N = 15,275) of lumbar spine volumetric BMD (vBMD) measured by quantitative computed tomography (QCT), allowing for examination of the trabecular bone compartment. SNPs that were significantly associated with vBMD were also examined in two GWAS meta-analyses to determine associations with morphometric vertebral fracture in (N = 21,701) and clinical vertebral fracture (N = 5,893). Expression QTL analyses of iliac crest biopsies were performed in 84 postmenopausal women, and murine osteoblast expression of genes implicated by eQTL or by proximity to vBMD-associated SNPs was examined. We identified significant vBMD associations with five loci, including 1p36.12, containing WNT4 and ZBTB40; 8q24, containing TNFRSF11B; and 13q14, containing AKAP11 and TNFSF11. Two loci (5p13 and 1p36.12) also contained associations with radiographic and clinical vertebral fracture, respectively. In 5p13, rs2468531 (minor allele frequency [MAF] = 3%) was associated with higher vBMD (β = 0.22, p = 1.9 × 10(-8) ) and decreased risk of radiographic vertebral fracture (OR = 0.75; false discovery rate [FDR] p = 0.01). In 1p36.12, rs12742784 (MAF = 21%) was associated with higher vBMD (β = 0.09, p = 1.2 × 10(-10) ) and decreased risk of clinical vertebral fracture (OR =0.82; FDR p = 7.4 × 10(-4) ). Both SNPs are non-coding and were associated with increased mRNA expression levels in human bone biopsies: rs2468531 with SLC1A3 (β = 0.28, FDR p = 0.01, involved in glutamate signaling and osteogenic response to mechanical loading) and rs12742784 with EPHB2 (β = 0.12, FDR p = 1.7 × 10(-3) , functions in bone-related ephrin signaling). Both genes are expressed in murine osteoblasts. This is the first study to link SLC1A3 and EPHB2 to clinically relevant vertebral osteoporosis phenotypes. These results may help elucidate vertebral bone biology and novel approaches to reducing vertebral fracture incidence. This article is protected by copyright. All rights reserved.

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