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Overexpression of the single-stranded DNA-binding protein (SSB) stabilises CAG center dot CTG triplet repeats in an orientation dependent manner

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalFEBS Letters
Volume584
Issue number1
DOIs
StatePublished - 4 Jan 2010

Abstract

The stability and deletion-size-distribution profiles of leading strand (CAG)(75) and (CTG)(137) trinucleotide repeat arrays inserted in the Escherichia coli chromosome were investigated upon overexpression of the single-stranded DNA-binding protein (SSB) and in mutant strains deficient for the SbcCD (Rad51/Mre11) nuclease. SSB overexpression increases the stability of the (CAG)(75) repeat array and leads to a loss of the bias towards large deletions for the same array. Furthermore, the absence of SbcCD leads to a reduction in the number of large deletions in strains containing the (CTG)(137) repeat array. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    Research areas

  • Trinucleotide repeat instability, Single-stranded DNA-binding protein, SbcCD, HUMAN GENETIC-DISEASE, ESCHERICHIA-COLI, TRINUCLEOTIDE REPEATS, DYNAMIC MUTATIONS, INSTABILITY, MECHANISMS, REPLICATION, CHROMOSOME, PALINDROME, EXPANSION

ID: 1487723