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Pax7 is regulated by cMyb during early neural crest development through a novel enhancer

Research output: Contribution to journalArticle

  • Stephanie Vadasz
  • Jonathan Marquez
  • Maria Tulloch
  • Natalia A Shylo
  • Martín I García-Castro

Related Edinburgh Organisations

Original languageEnglish
Pages (from-to)3691-702
Number of pages12
JournalDevelopment
Volume140
Issue number17
DOIs
Publication statusPublished - Sep 2013

Abstract

The neural crest (NC) is a migratory population of cells unique to vertebrates that generates many diverse derivatives. NC cells arise during gastrulation at the neural plate border (NPB), which is later elevated as the neural folds (NFs) form and fuse in the dorsal region of the closed neural tube, from where NC cells emigrate. In chick embryos, Pax7 is an early marker, and necessary component of NC development. Unlike other early NPB markers, which are co-expressed in lateral ectoderm, medial neural plate or posterior-lateral mesoderm, Pax7 early expression seems more restricted to the NPB. However, the molecular mechanisms controlling early Pax7 expression remain poorly understood. Here, we identify a novel enhancer of Pax7 in avian embryos that replicates the expression of Pax7 associated with early NC development. Expression from this enhancer is found in early NPB, NFs and early emigrating NC, but unlike Pax7, which is also expressed in mesodermal derivatives, this enhancer is not active in somites. Further analysis demonstrates that cMyb is able to interact with this enhancer and modulates reporter and endogenous early Pax7 expression; thus, cMyb is identified as a novel regulator of Pax7 in early NC development.

    Research areas

  • Animals, Chick Embryo, Electrophoretic Mobility Shift Assay, Electroporation, Enhancer Elements, Genetic, Gene Expression Regulation, Developmental, Immunohistochemistry, In Situ Hybridization, Mutagenesis, Site-Directed, Neural Crest, Neural Plate, PAX7 Transcription Factor

ID: 13281462