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Phospholipase C-beta 1 Signaling Affects Reproductive Behavior, Ovulation, and Implantation

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    Rights statement: Copyright © 2009 by The Endocrine Society

    Final published version, 193 KB, PDF document

Original languageEnglish
Pages (from-to)3259-3266
Number of pages8
Issue number7
Publication statusPublished - Jul 2009


Infertility can result from a wide range of defects, from behavioral, through germ cell development and maturation, to fertilization or embryo development. Many of the hormones regulating these processes signal via G protein-coupled receptors, which in turn activate a range of plasma membrane enzymes including phospholipase C (PLC)-beta isoforms. Transgenic mice lacking functional Plc-beta 1 (Plc-beta 1 KO mice) have been noted to have severely impaired fertility, but there has been little study of the reproductive processes affected by lack of this enzyme. This study examined reproductive behavior, gonadal development, fertilization, and implantation in Plc-beta 1 KO mice. Male and female Plc-beta 1 KO mice exhibited impaired reproductive behavior. No other defect in reproduction was noted in males, raising the possibility that the reduced fertility of Plc-beta 1 KO males could be due solely to impaired behavior. In contrast, female Plc-beta 1 KO mice exhibited both behavioral and nonbehavioral defects. Plc-beta 1 KO females ovulated only in response to exogenous hormones, with a large proportion of in vivo embryos recovered on embryonic d 4.5 exhibiting abnormal morphology. In addition, uteri of pregnant Plc-beta 1 KO females exhibited an implantation defect, with poor embryo attachment and a failure to up-regulate cyclooxygenase-2 mRNA.

    Research areas

  • Animals, Behavior, Animal/physiology, Cyclooxygenase 2/genetics, Embryo Implantation/physiology, Estrous Cycle/physiology, Female, Fertility, Fertilization in Vitro, Infertility, Female/genetics, Infertility, Female/veterinary, Male, Mice, Mice, Knockout, Ovulation/genetics, Ovulation/physiology, Phospholipase C beta/physiology, RNA, Messenger/metabolism, Reproduction/physiology, Signal Transduction/physiology

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