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Polymerizable Fluorescein Derivatives: Synthesis of Fluorescent Particles and Their Cellular Uptake

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Original languageEnglish
Pages (from-to)4386-4391
Number of pages6
JournalBiomacromolecules
Volume12
Issue number12
DOIs
Publication statusPublished - Dec 2011

Abstract

Fluorescent particles are used for a diverse number of biochemical assays including intracellular imaging, cellular tracking, as well as detection of a variety of biomolecules. They are typically prepared by postpolymerization conjugations of dyes onto preformed particles; Herein we report the synthesis of aminomethyl-functionalized fluorescent particles via the synthesis and application of polymerizable fluorescein monomers. These monomers allowed high and controllable fluorophore loading, into the particles, resulting in enhanced fluorescence properties in comparison with, more commonly. used carboxyfluorescein conjugated particles. Furthermore, the particles were rapidly taken up by cells with enhanced fluorescence. The herein presented results demonstrate the advantages Of dye polymerization in contrast with more conventional conjugation strategies for fluorescent particle generation with applications in the life sciences.

    Research areas

  • PHOTOINDUCED ELECTRON-TRANSFER, MINIEMULSION POLYMERIZATION, LIVING CELLS, NANOPARTICLES, MICROSPHERES, CHEMISTRY, DELIVERY, DESIGN, FLUOROPHORES, BIOMOLECULES

ID: 1463220