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Regulation of alternative polyadenylation by genomic imprinting

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  • Andrew Wood
  • Reiner Schulz
  • Kathryn Woodfine
  • Katarzyna Koltowska
  • Colin V Beechey
  • Jo Peters
  • Deborah Bourc'his
  • Rebecca J Oakey

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    Rights statement: Copyright © 2008, Cold Spring Harbor Laboratory Press

    Final published version, 629 KB, PDF document

http://genesdev.cshlp.org/content/22/9/1141
Original languageEnglish
Pages (from-to)1141-6
Number of pages6
JournalGenes & Development
Volume22
Issue number9
DOIs
Publication statusPublished - 2008

Abstract

Maternally and paternally derived alleles can utilize different promoters, but allele-specific differences in cotranscriptional processes have not been reported. We show that alternative polyadenylation sites at a novel murine imprinted gene (H13) are utilized in an allele-specific manner. A differentially methylated CpG island separates polyA sites utilized on maternal and paternal alleles, and contains an internal promoter. Two genetic systems show that alleles lacking methylation generate truncated H13 transcripts that undergo internal polyadenylation. On methylated alleles, the internal promoter is inactive and elongation proceeds to downstream polyadenylation sites. This demonstrates that epigenetic modifications can influence utilization of alternative polyadenylation sites.

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