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Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle

Research output: Contribution to journalArticle

  • Sabine E. Eckert
  • Francis Dziva
  • Roy R. Chaudhuri
  • Gemma C. Langridge
  • Daniel J. Turner
  • Derek J. Pickard
  • Duncan J. Maskell
  • Nicholas R. Thomson
  • Mark P. Stevens

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Original languageEnglish
Pages (from-to)1771-1776
Number of pages6
JournalJournal of Bacteriology
Issue number7
StatePublished - Apr 2011


Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.

    Research areas

  • Animals, Cattle, Cattle Diseases, Chromosomes, Bacterial, DNA Transposable Elements, DNA, Bacterial, Escherichia coli O157, Genetic Fitness, Genotype, Mutagenesis, Insertional, Plasmids

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