Edinburgh Research Explorer

Roles of neutrophils in the regulation of the extent of human inflammation through delivery of IL-1 and clearance of chemokines

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Open Access permissions

Open

Documents

  • Download as Adobe PDF

    Rights statement: © 2013 Society for Leukocyte Biology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/us/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Final published version, 3.84 MB, PDF document

    Licence: Creative Commons: Attribution Non-Commercial (CC-BY-NC)

http://www.jleukbio.org/content/93/1/7
Original languageEnglish
Pages (from-to)7-19
Number of pages13
JournalJournal of Leukocyte Biology
Volume93
Issue number1
DOIs
Publication statusPublished - Jan 2013

Abstract

This study examined the establishment of neutrophilic inflammation in humans. We tested the hypotheses that neutrophil recruitment was associated with local CXCL8 production and that neutrophils themselves might contribute to the regulation of the size of the inflammatory response. Humans were challenged i.d. with endotoxin. Biopsies of these sites were examined for cytokine production and leukocyte recruitment by qPCR and IHC. Additional in vitro models of inflammation examined the ability of neutrophils to produce and sequester cytokines relevant to neutrophilic inflammation. i.d. challenge with 15 ng of a TLR4-selective endotoxin caused a local inflammatory response, in which 1% of the total biopsy area stained positive for neutrophils at 6 h, correlating with 100-fold up-regulation in local CXCL8 mRNA generation. Neutrophils themselves were the major source of the early cytokine IL-1 beta. In vitro, neutrophils mediated CXCL8 but not IL-1 beta clearance (>90% clearance of

    Research areas

  • Toll-like receptor, endotoxin, neutrophil elastase, Interleukin-8, intradermal, NECROSIS-FACTOR-ALPHA, HUMAN POLYMORPHONUCLEAR LEUKOCYTES, RESPIRATORY-DISTRESS-SYNDROME, TOLL-LIKE RECEPTOR-2, IN-VIVO, CROSS-DESENSITIZATION, LUNG-DISEASE, ACTIVATION, EXPRESSION, CXCR1

Download statistics

No data available

ID: 17164398