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Safe and efficient in vitro and in vivo gene delivery: tripodal cationic lipids with programmed biodegradability

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)2154-2158
Number of pages5
JournalJournal of Materials Chemistry
Volume21
Issue number7
DOIs
Publication statusPublished - 2011

Abstract

The therapeutic use of nucleic acids has long been heralded as a panacea of medicinal opportunity, a vision enhanced by the introduction of RNA interference technology. The Achilles heel of such an approach is the in vivo delivery of the desired nucleic acid into cells, a practice that lacks selectivity, safety and/or efficiency. Herein we report the safe and efficacious in vitro and in vivo delivery of nucleic acids using tripodal biodegradable cationic lipids. Toxicity reduction and transfection potency of these novel amphiphiles were addressed by designing the compounds to undergo complete intracellular degradation thereby enhancing cargo release while minimising toxicity and potential tissue accumulation. Compounds demonstrated high-efficiency in transfecting DNA into cells both in vitro and in vivo with no signs of toxicity, thus potentially offering a safer alternative to viral transfection for gene therapy application.

    Research areas

  • MAMMALIAN-CELLS, GEMINI SURFACTANTS, TRANSFECTION, THERAPY, CARRIERS, DESIGN, DNA, AMPHIPHILES, LIPOSOME, VECTORS

ID: 1478337