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Scaffold based 3-D Cell Culture Imaging Using a Miniature Electrical Impedance Tomography Sensor

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Original languageEnglish
Pages (from-to)9071 - 9080
JournalIEEE Sensors Journal
Volume19
Issue number20
Early online date20 Jun 2019
DOIs
Publication statusPublished - 15 Oct 2019

Abstract

3-D Electrical Impedance Tomography (EIT) is an emerging technique for real-time and non-destructive 3-D cell culture imaging. This paper presents a pioneering study of scaffold-based 3-D cell culture imaging using a miniature planar EIT sensor. A 17-electrode miniature-planar EIT sensor equipped with a regular-shape 3-D printed scaffold was manufactured, modelled and characterized. In addition, an efficient 3-D image reconstruction method based on 3-D isotropic Total Variation and l1 joint regularization were proposed. Numerical simulation on scaffold phantoms and experimental study on time-varying distribution of MCF-7 cancer cell suspension within the scaffold were performed. Both simulation and experiment results suggest that using the miniature EIT sensor and the developed 3-D image reconstruction algorithms are able to achieve high quality, non-destructive scaffold-based 3-D cell culture imaging.

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