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Semi-automated Production of Hepatocyte like Cells from Pluripotent Stem Cells

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  • Jose Meseguer ripollés
  • Baltasar Lucendo-Villarin
  • Yu Wang
  • David Hay

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Original languageEnglish
Article numbere57995
JournalJournal of Visualized Experiments (JoVE)
Issue number137
DOIs
Publication statusPublished - 27 Jul 2018

Abstract

Human pluripotent stem cells represent a renewable source of human tissue. Our research is focused on generating human liver tissue from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). Current differentiation procedures generate human hepatocyte-like cells (HLCs) displaying a mixture of fetal and adult traits. To improve cell phenotype, we have fully defined our differentiation procedure and the cell niche, resulting in the generation of cell populations which display improved gene expression, function and phenotypic stability. While these studies mark progress, the ability to generate large quantities of multi well plates on HLCs for screening has been limited by labour intensive procedures which cause significant batch to batch variation. To tackle this issue, we have developed a semi-automated platform to differentiate pluripotent stem cells into HLCs. Stem cell seeding and differentiation were performed using liquid handling and automatic pipetting systems in a 96 well plate format. Following differentiation, cell phenotype was analyzed using automated microscopy and a multi well luminometer.

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