Edinburgh Research Explorer

Separating fluorescence from Raman spectra using a CMOS SPAD TCSPC line sensor for biomedical applications

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Original languageEnglish
Title of host publicationPaper 10873-26 Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis
PublisherSPIE
Publication statusPublished - 6 Feb 2019

Abstract

A recently developed CMOS SPAD line sensor is used to separate Raman scattering from background fluorescence without the use of time-gating, relying entirely on the in-pixel time-correlated single photon counting (TCSPC). The system delivers both Raman and time-resolved fluorescence decay data and is being developed with the aim of evaluating the suitability of liver tissue for transplant surgery as their high fluorescence emission across a wide spectral range poses particular challenges to commercial Raman systems. The system is benchmarked using samples of pure distilled-water and common organic fluorophores, in comparison with results from a Renishaw InVia spectrometer.

ID: 80289952