Edinburgh Research Explorer

Solid-Like Domains in Mixed Lipid Bilayers: Effect of Membrane Lamellarity and Transition Pathway

Research output: Chapter in Book/Report/Conference proceedingChapter

  • Vernita D. Gordon
  • Paul A. Beales
  • Gemma C. Shearman
  • Zhijun Zhao
  • John M. Seddon
  • Wilson C. K. Poon
  • Stefan U. Egelhaaf

Related Edinburgh Organisations

Original languageEnglish
Title of host publicationADVANCES IN PLANAR LIPID BILAYERS AND LIPOSOMES, VOL 20
EditorsA Iglic, CV Kulkarni
Place of PublicationSAN DIEGO
PublisherELSEVIER ACADEMIC PRESS INC
Pages137-154
Number of pages18
ISBN (Print)978-0-12-418698-9
DOIs
Publication statusPublished - 2014

Publication series

NameAdvances in Planar Lipid Bilayers and Liposomes
PublisherELSEVIER ACADEMIC PRESS INC
Volume20
ISSN (Print)1554-4516

Abstract

We present optical observations of phase separation in mixed model membranes in the form of giant unilamellar vesicles. These observations are compared to the phase behavior of lipid mixtures, which we determined by X-ray scattering and differential scanning calorimetry or extracted from the existing literature. The domain properties are affected not only by the bulk phase behavior but also by the membrane lamellarity and phase transition pathways. These observations have important implications for how phase behavior determined by bulk methods using dense, multilamellar lipid bilayers are linked to phase separation in giant, unilamellar lipid bilayers as observed by microscopy.

    Research areas

  • SUB-MAIN-TRANSITION, FLUORESCENCE CORRELATION SPECTROSCOPY, PHASE-TRANSITIONS, PHOSPHATIDYLETHANOLAMINE BILAYERS, LATERAL DIFFUSION, BINARY-MIXTURES, VESICLES, TEMPERATURE, MICROSCOPY, PHOSPHOLIPIDS

ID: 21294644