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Staphylococcus aureus lipase 1 enhances influenza A virus replication

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Original languageEnglish
JournalmBio
Early online date7 Jul 2020
DOIs
Publication statusE-pub ahead of print - 7 Jul 2020

Abstract

Influenza A virus (IAV) causes annual epidemics of respiratory disease in humans, often 31 complicated by secondary co-infection with bacterial pathogens such as Staphylococcus 32 aureus. Here, we report that the S. aureus secreted protein lipase 1 enhances IAV replication 33 in vitro in primary cells, including human lung fibroblasts. The pro-viral activity of lipase 1 34 is dependent on its enzymatic function, acts late in the viral lifecycle and results in increased 35 infectivity through positive modulation of virus budding. Furthermore, the pro-viral effect of 36 lipase 1 on IAV is exhibited during in vivo infection of embryonated hen’s eggs and 37 importantly, increases the yield of a vaccine strain of IAV by approximately 5-fold. Thus, we 38 have identified the first S. aureus protein to enhance IAV replication, suggesting a potential 39 role in co-infection. Importantly, this activity may be harnessed to address global shortages of 40 influenza vaccines.

    Research areas

  • Influenza A virus, Staphylococcus aureus, Co-infection, lipase 1

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