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Suppression of inflammatory immune responses in celiac disease by experimental hookworm infection

Research output: Contribution to journalArticle

  • Henry J McSorley
  • Soraya Gaze
  • James Daveson
  • Dianne Jones
  • Robert P Anderson
  • Andrew Clouston
  • Nathalie E Ruyssers
  • Richard Speare
  • James S McCarthy
  • Christian R Engwerda
  • John Croese
  • Alex Loukas

Related Edinburgh Organisations

Original languageEnglish
Pages (from-to)e24092
JournalPLoS ONE
Volume6
Issue number9
DOIs
Publication statusPublished - 2011

Abstract

We present immunological data from two clinical trials where the effect of experimental human hookworm (Necator americanus) infection on the pathology of celiac disease was evaluated. We found that basal production of Interferon- (IFN-)γ and Interleukin- (IL-)17A from duodenal biopsy culture was suppressed in hookworm-infected participants compared to uninfected controls. Increased levels of CD4+CD25+Foxp3+ cells in the circulation and mucosa are associated with active celiac disease. We show that this accumulation also occurs during a short-term (1 week) oral gluten challenge, and that hookworm infection suppressed the increase of circulating CD4+CD25+Foxp3+ cells during this challenge period. When duodenal biopsies from hookworm-infected participants were restimulated with the immunodominant gliadin peptide QE65, robust production of IL-2, IFN-γ and IL-17A was detected, even prior to gluten challenge while participants were strictly adhering to a gluten-free diet. Intriguingly, IL-5 was produced only after hookworm infection in response to QE65. Thus we hypothesise that hookworm-induced TH2 and IL-10 cross-regulation of the TH1/TH17 inflammatory response may be responsible for the suppression of these responses during experimental hookworm infection.

    Research areas

  • Animals, Antigens, CD4, Biopsy, Celiac Disease, Cells, Cultured, Clinical Trials as Topic, Duodenum, Forkhead Transcription Factors, Gliadin, Host-Parasite Interactions, Humans, Immunohistochemistry, Interferon-gamma, Interleukin-17, Interleukin-2, Interleukin-2 Receptor alpha Subunit, Interleukin-5, Intestinal Mucosa, Leukocytes, Mononuclear, Necator americanus, Necatoriasis, Time Factors

ID: 29032274