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Surface functionalization affects the zeta potential, coronal stability and membranolytic activity of polymeric nanoparticles

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)202-211
Number of pages10
JournalNanotoxicology
Volume8
Issue number2
DOIs
Publication statusPublished - Mar 2014

Abstract

Nano materials are commonly functionalized to boost their physicochemical properties. However, there is little known about the impact of these modifications on cellular systems. Herein, we synthesized eight types of polymeric nanoparticles (NPs) bearing different functional groups, and investigated their effects on interactions with cellular membranes. As models for particle membrane interactions, hemolysis assays using human red blood cells and culture with A549 cells were utilized. Under protein-free conditions, the NPs showed a wide distribution of zeta potentials (ζPs) which showed a good correlation with their hemolytic potential. However, in the presence of serum or lung lining fluid, the ζPs of all NPs coalesced towards a single common negative value and showed neither hemolytic activity nor cytotoxicity to A549 cells. Lipase and protease treatment of the coronated particles did not restore their reactivity. These result simply proves that particle functionalization influences the stability of the particle corona which, if intact, prevents hemolytic activity and membrane disrupture.

    Research areas

  • corona, hemolysis, polymeric nanoparticles, surface functionalization, toxicity, zeta potential, CAUSE PULMONARY INFLAMMATION, METAL-OXIDE NANOPARTICLES, NLRP3 INFLAMMASOME, PROTEIN ADSORPTION, IN-VITRO, CELLS, ACTIVATE, LUNG, CHALLENGES, PARTICLES

ID: 14125213