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Targeted genome editing across species using ZFNs and TALENs

Research output: Contribution to journalArticle

  • Andrew Wood
  • Te-Wen Lo
  • Bryan Zeitler
  • Catherine S Pickle
  • Edward J Ralston
  • Andrew H Lee
  • Rainier Amora
  • Jeffrey C Miller
  • Elo Leung
  • Xiangdong Meng
  • Lei Zhang
  • Edward J Rebar
  • Philip D Gregory
  • Fyodor D Urnov
  • Barbara J Meyer

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http://www.sciencemag.org/content/333/6040/307
Original languageEnglish
Pages (from-to)307
JournalScience
Volume333
Issue number6040
DOIs
Publication statusPublished - 15 Jul 2011

Abstract

Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.

    Research areas

  • Animals, Regulatory Elements, Transcriptional, Transgenes, Genome, Helminth, INDEL Mutation, Mutagenesis, Caenorhabditis, Transcription Factors, Genetic Techniques, Caenorhabditis elegans, Zinc Fingers, Deoxyribonucleases, Type II Site-Specific, Genes, Helminth, Gene Targeting

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