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Temporal profiles of appearance of DNase I hypersensitive sites associated with the ovine beta-lactoglobulin gene differ in sheep and transgenic mice

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Original languageEnglish
Pages (from-to)649-54
Number of pages6
JournalMolecular & general genetics
Issue number6
Publication statusPublished - Apr 1998


The ovine milk protein beta-lactoglobulin is expressed in a distinct temporal pattern during lactogenesis. This expression pattern is reflected in the temporal profile of appearance of DNase I hypersensitive sites (HS) associated with the beta-lactoglobulin gene in the mammary gland. Specifically, HSIV and HSV are present prior to the first major increase in expression, which occurs at mid-pregnancy, while HSI displays the converse profile, being detected after mid-pregnancy and during lactation. The extent of DNase I digestion at HSIII, encompassing the promoter region, reflects the level of beta-lactoglobulin expression. In transgenic mouse mammary chromatin, beta-lactoglobulin transgenes display the same set of DNase I hypersensitive sites as in sheep mammary chromatin. The temporal profile, however, differs from that seen in sheep: notably, HSIV and HSV are detected during lactation. The fact that beta-lactoglobulin transgenes lacking HSIV and HSV are expressed but display a reduced transcription rate per integrated copy is compatible with a functional role for these regions. This suggests that HSIV and HSV may increase the likelihood of high-level transgene expression.

    Research areas

  • Animals, Deoxyribonuclease I, Female, Gene Deletion, Gene Expression, Humans, Lactoglobulins, Mice, Mice, Transgenic, Pregnancy, Sheep, Species Specificity, Transcription, Genetic, Transgenes

ID: 8777416