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Temporal profiling of the coding and noncoding murine cytomegalovirus transcriptomes

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    Rights statement: Available online: http://jvi.asm.org/content/85/12/6065 Copyright © 2013 by the American Society for Microbiology.

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http://jvi.asm.org/content/85/12/6065
Original languageEnglish
Pages (from-to)6065-6076
Number of pages12
JournalJournal of Virology
Volume85
Issue number12
DOIs
Publication statusPublished - Jun 2011

Abstract

The global transcriptional program of murine cytomegalovirus (MCMV), involving coding, noncoding, and antisense transcription, remains unknown. Here we report an oligonucleotide custom microarray platform capable of measuring both coding and noncoding transcription on a genome-wide scale. By profiling MCMV wild-type and immediate-early mutant strains in fibroblasts, we found rapid activation of the transcriptome by 6.5 h postinfection, with absolute dependency on ie3, but not ie1 or ie2, for genomic programming of viral gene expression. Evidence is also presented to show, for the first time, genome-wide noncoding and bidirectional transcription at late stages of MCMV infection.

    Research areas

  • Animals, Fibroblasts, Gene Expression Profiling, Gene Expression Regulation, Viral, Genome, Viral, Herpesviridae Infections, Immediate-Early Proteins, Mice, Muromegalovirus, Mutation, NIH 3T3 Cells, Oligonucleotide Array Sequence Analysis, Time Factors, Viral Proteins

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