Edinburgh Research Explorer

The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Open Access permissions

Open

Documents

  • Download as Adobe PDF

    Rights statement: Use of open access articles is permitted based on the terms of the specific Creative Commons Licence under with the article is published. archiving of non-open access articles is permitted in accordance with the Archiving Policy of Portland Press (http://www.portlandpresspublishing.com/content/open-access-policy#archiving).

    Accepted author manuscript, 1 MB, PDF-document

  • Download as Adobe PDF

    Rights statement: © 2019 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).

    Final published version, 1 MB, PDF-document

    Licence: Creative Commons: Attribution (CC-BY)

Original languageEnglish
Pages (from-to)179-191
Number of pages13
JournalBiochemical Journal
Volume476
Issue number2
Early online date7 Nov 2018
DOIs
Publication statusPublished - 18 Jan 2019

Abstract

Eukaryotic ATP-dependent phosphofructokinases (PFKs) are often considered unidirectional enzymes catalysing the transfer of a phospho moiety from ATP to fructose 6-phosphate (F6P) to produce ADP and fructose 1,6-bisphosphate (F16BP). The reverse reaction is not generally considered to occur under normal conditions and has never been demonstrated for any eukaryotic ATP-dependent PFKs, though it does occur in PPi-dependent PFKs and has been experimentally shown for bacterial ATP-dependent PFKs. Evidence is provided via two orthogonal assays that all three human PFK isoforms can catalyse the reverse reaction in vitro , allowing determination of kinetic properties. Additionally, the reverse reaction was shown possible for PFKs from three clinically important trypanosomatids; these enzymes are contained within glycosomes in vivo This compartmentalisation may facilitate reversal, given the potential for trypanosomatids to have an altered ATP/ADP ratio in glycosomes compared to the cytosol. The kinetic properties of each trypanosomatid PFK were determined, including the response to natural and artificial modulators of enzyme activity. The possible physiological relevance of the reverse reaction in trypanosomatid and human PFKs is discussed.

    Research areas

  • Phosphofructokinase, Human, Trypanosoma cruzi, Trypanosoma brucei, Leishmania, Trypanosomatid, Reverse reaction

Download statistics

No data available

ID: 77702620