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The Nrd1-like protein Seb1 coordinates cotranscriptional 3′ end processing and polyadenylation site selection

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  • Jean François Lemay
  • Samuel Marguerat
  • Marc Larochelle
  • Xiaochuan Liu
  • Rob van Nues
  • Judit Hunyadkürti
  • Mainul Hoque
  • Bin Tian
  • Sander Granneman
  • Jürg Bähler
  • François Bachand

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    Rights statement: © 2016 Lemay et al. This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http:// creativecommons.org/licenses/by-nc/4.0/

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Original languageEnglish
Pages (from-to)1558-1572
Number of pages15
JournalGenes & Development
Volume30
Issue number13
DOIs
Publication statusPublished - 1 Jul 2016

Abstract

Termination of RNA polymerase II (RNAPII) transcription is associated with RNA 3′ end formation. For coding genes, termination is initiated by the cleavage/polyadenylation machinery. In contrast, a majority of noncoding transcription events in Saccharomyces cerevisiae does not rely on RNA cleavage for termination but instead terminates via a pathway that requires the Nrd1–Nab3–Sen1 (NNS) complex. Here we show that the Schizosaccharomyces pombe ortholog of Nrd1, Seb1, does not function in NNS-like termination but promotes polyadenylation site selection of coding and noncoding genes. We found that Seb1 associates with 3′ end processing factors, is enriched at the 3′ end of genes, and binds RNA motifs downstream from cleavage sites. Importantly, a deficiency in Seb1 resulted in widespread changes in 3′ untranslated region (UTR) length as a consequence of increased alternative polyadenylation. Given that Seb1 levels affected the recruitment of conserved 3′ end processing factors, our findings indicate that the conserved RNA-binding protein Seb1 cotranscriptionally controls alternative polyadenylation.

    Research areas

  • 3′ end processing, Nrd1, Polyadenylation, S. pombe, Seb1, Transcription termination

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