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The SNF2 family ATPase LSH promotes cell-autonomous de novo DNA methylation in somatic cells

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Original languageEnglish
JournalNucleic Acids Research
Early online date13 May 2016
DOIs
StatePublished - 19 Sep 2016

Abstract

Methylation of DNA at carbon 5 of cytosine is essential for mammalian development and implicated in transcriptional repression of genes and transposons. New patterns of DNA methylation characteristic of lineage-committed cells are established at the exit from pluripotency by de novo DNA methyltransferases enzymes, DNMT3A and DNMT3B, which are regulated by developmental signalling and require access to chromatin-organised DNA. Whether or not the capacity for de novo DNA methylation of developmentally regulated loci is preserved in differentiated somatic cells and can occur in the absence of exogenous signals is currently unknown. Here we demonstrate that fibroblasts derived from chromatin remodelling ATPase LSH (HELLS)-null mouse embryos, which lack DNA methylation from centromeric repeats, transposons and a number of gene promoters, are capable of re-establishing DNA methylation and silencing of miss-regulated genes upon re-expression of LSH. We also show that the ability of LSH to bind ATP and the cellular concentration of DNMT3B are critical for cell-autonomous de novo DNA methylation in somatic cells. These data suggest the existence of cellular memory that persists in differentiated cells through many cell generations and changes in transcriptional state.

    Research areas

  • epigenetics, DNA methylation, chromatin, gene silencing, LSH

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