Edinburgh Research Explorer

Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)1371-5
Number of pages5
JournalBiomaterials science
Volume3
Issue number10
DOIs
Publication statusPublished - 15 Oct 2015

Abstract

A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N'-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a "naïve" state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.

Download statistics

No data available

ID: 22273478