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Use of broth cultures peri-operatively to optimise the microbiological diagnosis of musculoskeletal implant infections

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)1566-1570
Number of pages5
JournalBone and Joint Journal
Issue number11
Publication statusPublished - Nov 2014


We compared the use of broth culture medium for samples taken in theatre with the standard practice of placing tissue samples in universal containers. A total of 67 consecutive patients had standard multiple samples of deep tissue harvested at surgery and distributed equally in theatre either to standard universal containers or to broth culture medium. These samples were cultured by direct and enrichment methods. The addition of broth in theatre to standard practice led to an increase in sensitivity from 83% to 95% and an increase in negative predictive value from 77% to 91%. Placing tissue samples directly into broth in the operating theatre is a simple, inexpensive way to increase the sensitivity of cultures from infected patients, and does not appear to compromise the specificity of these cultures.

    Research areas

  • Bacteria, Culture Media, Diagnosis, Differential, Humans, Musculoskeletal Diseases, Orthopedic Procedures, Perioperative Period, Predictive Value of Tests, Prostheses and Implants, Prosthesis-Related Infections, ROC Curve

ID: 18017331