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Vaccinia virus kelch protein A55 is a 64 kDa intracellular factor that affects virus-induced cytopathic effect and the outcome of infection in a murine intradermal model

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Original languageEnglish
Pages (from-to)1521-9
Number of pages9
JournalJournal of General Virology
Volume87
Issue numberPt 6
DOIs
StatePublished - Jun 2006

Abstract

The vaccinia virus (VACV) protein A55 is a BTB/kelch protein with a broad-complex, tramtrack and bric-a-brac (BTB) domain in the N-terminal region and five kelch repeats in the C-terminal half. The BTB/kelch subgroup of the kelch superfamily of proteins has been associated with a wide variety of functions including regulation of the cytoskeleton. VACV contains three genes predicted to encode BTB/kelch proteins: A55R, F3L and C2L. The A55R gene product has been identified as an intracellular protein of 64 kDa that is expressed late in infection. A VACV strain lacking 93.6% of the A55R open reading frame (vdeltaA55) was constructed and found to have an unaltered growth rate in vivo but a different plaque morphology and cytopathic effect, as well as reduced development of VACV-induced Ca2+-independent cell/extracellular matrix adhesion. In a murine intradermal model of VACV infection, a virus lacking the A55R gene induced larger lesions than wild-type and revertant control viruses.

    Research areas

  • Animals, Carrier Proteins, Cell Line, Cytopathogenic Effect, Viral, Disease Models, Animal, Female, Humans, Mice, Mice, Inbred BALB C, Skin Diseases, Viral, Vaccinia, Vaccinia virus, Viral Proteins, Virulence

ID: 8533185